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1st appeared
05
December
2000
Finding Could Lead to New Approach for Treating Severe Heart Disease UCSF researchers report that a new approach for delivering the potent growth factor VEGF into mice with coronary heart disease prompted the growth of blood vessels in damaged heart tissue, without causing side effects, offering hope for a treatment strategy for coronary heart disease that until now has met with setbacks. The finding is reported in the current issue of Proceedings of the National Academy of Science. VEGF, or vascular endothelial growth factor, induces the growth of blood vessels throughout the body, and researchers believe that it could be used therapeutically to induce blood vessel growth, or angiogenesis, in heart muscle that has been damaged by lack of oxygen, as occurs in coronary heart disease. Increased blood flow, and thus oxygen, into heart muscle could rejuvenate oxygen-deprived cells. This kind of treatment for severe coronary heart disease could prove valuable, because while present treatments – angioplasty, a procedure that involves cleaning out clogged arteries, and coronary bypass surgery – are often helpful, renarrowing, or restinosis, of coronary vessels occurs in 30 to 35 percent of cases. Numerous techniques for introducing VEGF into failing heart muscle in animals and humans have prompted blood vessel formation and improved circulation in damaged tissue. However, each strategy has had short-lasting effects, caused an immunologic reaction, or led to the formation of angiomas, or tumors. In previous human studies, injection of VEGF protein into ailing heart muscle caused only a fleeting angiogenic response, as a protein’s life span is short. Injection of the VEGF gene via a molecule of DNA known as a plasmid, caused the development of angiomas. And insertion of the VEGF gene via a common cold virus known as adenovirus prompted an immunologic reaction in heart muscle cells, leading to inflammation, which in turn causes arrhythmia, or abnormal heart rhythm. In the UCSF study, researchers used an adeno-associated viral vector to transport a human form of the VEGF gene into the heart muscle of mice. The vector was injected into several sites of damaged heart muscle, as well as into one site of normal, healthy heart muscle. Unlike adenovirus, adeno-associated virus is not known to cause any human disease. The VEGF gene was expressed in normal and damaged tissues. But VEGF protein, produced by the gene, prompted the growth of new blood vessels only in damaged tissue. Notably, the adeno-associated viral vector did not prompt an inflammatory cell response, or the development of angioma-like structures. "These results indicate that the adeno-associated viral vector may be an ideal mode for delivering the VEGF gene into the heart muscle," says the lead author of the study, Hua Su, MD, assistant research physician in the UCSF Cardiovascular Research Institute and a member of the UCSF laboratory of senior author Yuet Wai Kan, MD, DSc, Louis K. Diamond Professor of Hematology, UCSF professor of laboratory medicine, and a Howard Hughes Medical Institute Investigator. "The finding demonstrates that the adeno-associated virus vector can mediate efficient gene transfer and adequate gene expression, and that VEGF gene delivered by this mechanism can induce angiogenesis in damaged heart muscle. Plasmid and adenoviral vectors, by contrast, apparently cause overly high expression of the VEGF gene." Links: Proceedings of the National Academy of Science Howard Hughes Medical Institute Source: Jennifer O'Brien, News Services |
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